Difference between revisions of "Part:BBa K5115061"
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===Usage and Biology===
 
===Usage and Biology===
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The ribozyme-assisted polycistronic co-expression system can ensure that each cistron can initiate translation with comparable efficiency. For more information, please check [https://2022.igem.wiki/fudan/parts part wiki of 2022 Fudan iGEM].
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Binding hoxF and EP can tether hydrogenase genes to the α-carboxysome, providing a protective microenvironment for the hydrogenase enzyme, enhancing its catalytic activity and stability. The ribozyme-assisted polycistronic co-expression system can ensure that each cistron can initiate translation with comparable efficiency. For more information, please check [https://2022.igem.wiki/fudan/parts part wiki of 2022 Fudan iGEM].
  
  

Revision as of 14:42, 28 September 2024


ribozyme+RBS+hoxF-GS-EP+stem-loop

contributed by Fudan iGEM 2023

Introduction

This composite part is composed of hoxF-GS-EP and improved ribozyme-assisted polycistronic co-expression system: pRAP. Get details about hoxF in BBa_K5115020(hox and hyp operon) and EP in BBa_K5115002(EP).

Usage and Biology

Binding hoxF and EP can tether hydrogenase genes to the α-carboxysome, providing a protective microenvironment for the hydrogenase enzyme, enhancing its catalytic activity and stability. The ribozyme-assisted polycistronic co-expression system can ensure that each cistron can initiate translation with comparable efficiency. For more information, please check part wiki of 2022 Fudan iGEM.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 654
    Illegal BglII site found at 1472
    Illegal BglII site found at 1765
    Illegal XhoI site found at 99
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 533
    Illegal NgoMIV site found at 1071
    Illegal NgoMIV site found at 1281
    Illegal NgoMIV site found at 1593
    Illegal AgeI site found at 2548
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 702
    Illegal BsaI.rc site found at 1206


References