Difference between revisions of "Part:BBa K079032:Experience"
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Dh5alpha cells transformed with BBa_K079032 and BBa_K079031 were inoculated  
 
Dh5alpha cells transformed with BBa_K079032 and BBa_K079031 were inoculated  
 
in M9 medium at 8.00 p.m. after O/N growth (about 12 h), samples were collected and slide prepared for image acquisition with the VIFluoR software.  
 
in M9 medium at 8.00 p.m. after O/N growth (about 12 h), samples were collected and slide prepared for image acquisition with the VIFluoR software.  
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[[Image:GrowthCurve1.png|center|600px |thumb|Fig.1 - Growth curve]]
 
[[Image:GrowthCurve1.png|center|600px |thumb|Fig.1 - Growth curve]]
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[[Image:FluorescenceCurveAbsolute1.png|center|600px |thumb|Fig.2 - Fluorescence]]
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[[Image:FluorescenceCurveAbsolute1.png|center|600px |thumb|Fig.2 - Absolute Fluorescence Curve]]
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[[Image:FluorescenceCurveOverOD1.png|center|600px |thumb|Fig.3 - Fluorescence curve over OD]]
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[[Image:FluorescenceCurveOverOD1.png|center|600px |thumb|Fig.3 - Fluorescence vs OD curve]]
  
 
As it can be seen from the figures above, data from the fluorimeter analysis agreed with the microscope image analysis. Indeed, the promoter fluorescence ratio was about 1.2.
 
As it can be seen from the figures above, data from the fluorimeter analysis agreed with the microscope image analysis. Indeed, the promoter fluorescence ratio was about 1.2.

Revision as of 20:10, 21 October 2009

Dh5alpha cells transformed with BBa_K079032 and BBa_K079031 were inoculated in M9 medium at 8.00 p.m. after O/N growth (about 12 h), samples were collected and slide prepared for image acquisition with the VIFluoR software. The BBa_K079032/ BBa_K079031 fluorescence ratio was equal to 1.20±0.4 (Table 1).

Table 1 - Promoter fluorescence ratio after microscope analysis

The same samples were diluited to an OD equal 0.1 and a growth in time was performed with a Tekcan spectrofluorimeter. Both optical density (OD) and fluorescence level were analized for 12 h (Fig.1 and Fig.2, respectively). Fluorescence was then normalized on the OD value (Fig.3).

Fig.1 - Growth curve
Fig.2 - Absolute Fluorescence Curve
Fig.3 - Fluorescence vs OD curve

As it can be seen from the figures above, data from the fluorimeter analysis agreed with the microscope image analysis. Indeed, the promoter fluorescence ratio was about 1.2.

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