Difference between revisions of "Part:BBa K5115070"
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bound AAA+ protease, and its protease activity toward LpxC is regulated by
 
bound AAA+ protease, and its protease activity toward LpxC is regulated by
 
essential membrane proteins LapB and YejM. YejM can compete with FtsH for LapB to inhibit LpxC
 
essential membrane proteins LapB and YejM. YejM can compete with FtsH for LapB to inhibit LpxC
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degradation, thus guaranteed the expression level of LPS.<ref>Shu, S., & Mi, W. (2022). Regulatory mechanisms of lipopolysaccharide synthesis in Escherichia coli. Nature Communications, 13(1), 4576.</ref>
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degradation, thus guaranteed the expression level of LPS.<ref>Shu, S., & Mi, W. (2022). Regulatory mechanisms of lipopolysaccharide synthesis in Escherichia coli. Nature Communications, 13(1), 4576.</ref> LPS is a basic component of ''E.coli'''s outer membrane. Sufficient expressing of LPS can help with the bacteria's survival, so, as a result,  the YejM can indirectly improve ''E.coli'''s viability.
  
 
===Usage and Biology===
 
===Usage and Biology===
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Our MINERAL is designed to be put into half-open water. To ensure that it can function stably, we choose to use YejM to raise ''E.coli'''s viability.
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===Characterization===
 
===Characterization===
  

Revision as of 14:01, 28 September 2024


YejM contributed by Fudan iGEM 2023

Introduction

YejM is an anti-adaptor protein that can regulate the synthesis of the LPS. In E.coli, LPS levels are under feedback control, achieved by FtsH-mediated degradation of LpxC. FtsH is a membrane- bound AAA+ protease, and its protease activity toward LpxC is regulated by essential membrane proteins LapB and YejM. YejM can compete with FtsH for LapB to inhibit LpxC degradation, thus guaranteed the expression level of LPS.[1] LPS is a basic component of E.colis outer membrane. Sufficient expressing of LPS can help with the bacteria's survival, so, as a result, the YejM can indirectly improve E.colis viability.

Usage and Biology

Our MINERAL is designed to be put into half-open water. To ensure that it can function stably, we choose to use YejM to raise E.coli's viability.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1440
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. ↑ Shu, S., & Mi, W. (2022). Regulatory mechanisms of lipopolysaccharide synthesis in Escherichia coli. Nature Communications, 13(1), 4576.