Difference between revisions of "Part:BBa K5269008"

 
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<partinfo>BBa_K5269008 short</partinfo>
 
<partinfo>BBa_K5269008 short</partinfo>
  
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This is a reverse primer used for PCR of the surface-presenting protein-SpyTag complex (BBa_K5269021-BBa_K5269024). This primer is also used for Golden Gate Assembly (GGA) to pBluescript II SK(-) which has Golden Gate Assembly Site (BBa_K5269020). This primer is used for GGA of three surface-presenting protein-SpyTag complexes, and is closest to the tac promoter.
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This is a reverse primer used for PCR of the Surface displayed protein-SpyTag complex (BBa_K5269021-BBa_K5269024). This primer is also used for Golden Gate Assembly (GGA) to pBluescript II SK(-) which has Golden Gate Assembly Site (BBa_K5269020). This primer is used for GGA of three Surface displayed protein-SpyTag complexes, and is closest to the tac promoter(BBa_K5269033).
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 03:31, 29 September 2024


Surface displayed protein reverse primer 4

This is a reverse primer used for PCR of the Surface displayed protein-SpyTag complex (BBa_K5269021-BBa_K5269024). This primer is also used for Golden Gate Assembly (GGA) to pBluescript II SK(-) which has Golden Gate Assembly Site (BBa_K5269020). This primer is used for GGA of three Surface displayed protein-SpyTag complexes, and is closest to the tac promoter(BBa_K5269033).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]