Difference between revisions of "Part:BBa K5199004"

Revision as of 22:51, 27 September 2024

INP Silicatein

Ice Nucleation Protein(INP)- Silicatein Fusion Protein

INP is a transmembrane protein that acts as an external display marker. INP fusion proteins can be successfully expressed on the outside of E. coli. INP has been used by other teams (K409000 and K4578009), here we combined INP with a truncated silicatein alpha gene (BBa_K5199003) which polymerizes and precipitate silica. With external silicatein expression, we are able to precipitate silica on the outside of E. coli.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 321
Illegal XhoI site found at 1246
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 63
Illegal NgoMIV site found at 396
Illegal AgeI site found at 498
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 765
Illegal SapI.rc site found at 229

@@ Line 5: / Line 5: @@
 Ice Nucleation Protein(INP)- Silicatein Fusion Protein
-INP is a transmembrane protein that acts as an external display marker. INP fusion proteins can be successfully expressed on the outside of E. coli. INP has been used by other teams (K409000 and K4578009), here we combined INP with a truncated silicatein alpha gene which polymerizes and precipitate silica. With external silicatein expression, we are able to precipitate silica on the outside of E. coli.
+INP is a transmembrane protein that acts as an external display marker. INP fusion proteins can be successfully expressed on the outside of E. coli. INP has been used by other teams (K409000 and K4578009), here we combined INP with a truncated silicatein alpha gene (BBa_K5199003) which polymerizes and precipitate silica. With external silicatein expression, we are able to precipitate silica on the outside of E. coli.
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