Difference between revisions of "Part:BBa K5109021"
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<partinfo>BBa_K5109020 short</partinfo>
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This composite part is identical to BBa_K5109023, except that the gene for the desired surface expression has been substituted with LacE (BBa_K5109014). This modification can be achieved using cloning techniques, specifically by digesting the surface expression cassette BBa_K5109023 with BsaI and BamHI, followed by ligation with the desired enzyme. To do this, specific BsaI and BamHI restriction sites must be added to the enzyme’s sequence.
 
This composite part is identical to BBa_K5109023, except that the gene for the desired surface expression has been substituted with LacE (BBa_K5109014). This modification can be achieved using cloning techniques, specifically by digesting the surface expression cassette BBa_K5109023 with BsaI and BamHI, followed by ligation with the desired enzyme. To do this, specific BsaI and BamHI restriction sites must be added to the enzyme’s sequence.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K5109020 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K5109021 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K5109020 parameters</partinfo>
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<partinfo>BBa_K5109021 parameters</partinfo>
 
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Revision as of 16:44, 27 September 2024

Laccase E surface display system

This composite part is identical to BBa_K5109023, except that the gene for the desired surface expression has been substituted with LacE (BBa_K5109014). This modification can be achieved using cloning techniques, specifically by digesting the surface expression cassette BBa_K5109023 with BsaI and BamHI, followed by ligation with the desired enzyme. To do this, specific BsaI and BamHI restriction sites must be added to the enzyme’s sequence.

Usage and Biology

Usage and Biology

This surface display system aims to express the laccase on the outer side of the cell membrane. This way, we could test it's functioning on PFAS molecules directly on the external medium, without stressing or damaging E. coli by expressing the enzyme inside.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 639
    Illegal BamHI site found at 2245
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 900
    Illegal NgoMIV site found at 1121
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 667