Difference between revisions of "Part:BBa K5109020"
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This composite part is identical to BBa_K5109023, except that the gene for the desired surface expression has been substituted with DehaS (BBa_K5109013). | This composite part is identical to BBa_K5109023, except that the gene for the desired surface expression has been substituted with DehaS (BBa_K5109013). | ||
This modification can be achieved using cloning techniques, specifically by digesting the surface expression cassette BBa_K5109023 with BsaI and BamHI, followed by ligation with the desired enzyme. | This modification can be achieved using cloning techniques, specifically by digesting the surface expression cassette BBa_K5109023 with BsaI and BamHI, followed by ligation with the desired enzyme. | ||
To do this, specific BsaI and BamHI restriction sites must be added to the enzyme’s sequence. | To do this, specific BsaI and BamHI restriction sites must be added to the enzyme’s sequence. | ||
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| + | ===Usage and Biology=== | ||
| + | Usage and Biology | ||
This surface display system aims to express the dehalogenase on the outer side of the cell membrane. This way, we could test it's functioning on PFAS molecules directly on the external medium, without stressing or damaging E. coli by expressing the enzyme inside. | This surface display system aims to express the dehalogenase on the outer side of the cell membrane. This way, we could test it's functioning on PFAS molecules directly on the external medium, without stressing or damaging E. coli by expressing the enzyme inside. | ||
Revision as of 16:41, 27 September 2024
Dehalogenase S surface display system
This composite part is identical to BBa_K5109023, except that the gene for the desired surface expression has been substituted with DehaS (BBa_K5109013). This modification can be achieved using cloning techniques, specifically by digesting the surface expression cassette BBa_K5109023 with BsaI and BamHI, followed by ligation with the desired enzyme. To do this, specific BsaI and BamHI restriction sites must be added to the enzyme’s sequence.
Usage and Biology
Usage and Biology
This surface display system aims to express the dehalogenase on the outer side of the cell membrane. This way, we could test it's functioning on PFAS molecules directly on the external medium, without stressing or damaging E. coli by expressing the enzyme inside.