Difference between revisions of "Part:BBa K5109020"
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| − | + | This composite part is identical to BBa_K5109023, except that the gene for the desired surface expression has been substituted with DehaS (BBa_K5109013). | |
| + | This modification can be achieved using cloning techniques, specifically by digesting the surface expression cassette BBa_K5109023 with BsaI and BamHI, followed by ligation with the desired enzyme. | ||
| + | To do this, specific BsaI and BamHI restriction sites must be added to the enzyme’s sequence. | ||
Revision as of 16:01, 27 September 2024
This composite part is identical to BBa_K5109023, except that the gene for the desired surface expression has been substituted with DehaS (BBa_K5109013). This modification can be achieved using cloning techniques, specifically by digesting the surface expression cassette BBa_K5109023 with BsaI and BamHI, followed by ligation with the desired enzyme. To do this, specific BsaI and BamHI restriction sites must be added to the enzyme’s sequence.