Difference between revisions of "Part:BBa K5143023"
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Our project aims to create a fully bio-sourced and biodegradable insect trap. To achieve this, the base of our trap will be made of cellulose produced by a bacterium in a co-culture system. The other organism in this co-culture is <i>Saccharomyces cerevisiae</i>, a yeast that will secrete a yellow chromoprotein and a bioglue (<a href="https://parts.igem.org/Part:BBa_K5143022">BBa_K5143022</a>). The adhesion of the yellow chromoprotein to the cellulose, which forms the base of our trap, will make it coloured, trapping insects that are specifically attracted to yellow.<br> | Our project aims to create a fully bio-sourced and biodegradable insect trap. To achieve this, the base of our trap will be made of cellulose produced by a bacterium in a co-culture system. The other organism in this co-culture is <i>Saccharomyces cerevisiae</i>, a yeast that will secrete a yellow chromoprotein and a bioglue (<a href="https://parts.igem.org/Part:BBa_K5143022">BBa_K5143022</a>). The adhesion of the yellow chromoprotein to the cellulose, which forms the base of our trap, will make it coloured, trapping insects that are specifically attracted to yellow.<br> | ||
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To allow the secretion and the cellulose binding of the fwYellow, two elements are fused to the fwYellow gene :<br> | To allow the secretion and the cellulose binding of the fwYellow, two elements are fused to the fwYellow gene :<br> | ||
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<div class="image-caption">The Yellow chromoprotein is secreted by <i>Saccharomyces cerevisiae</i>, then it binds to the cellulose to functionalize it.</div> | <div class="image-caption">The Yellow chromoprotein is secreted by <i>Saccharomyces cerevisiae</i>, then it binds to the cellulose to functionalize it.</div> | ||
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<h1>Construction</h1> | <h1>Construction</h1> | ||
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This sequence was designed to be fully optimized for expression in <i>Saccharomyces cerevisiae</i> and for synthesis. This fragment was then cloned with the bioglue fragment (<a href="https://parts.igem.org/Part:BBa_K5143022">BBa_K5143022</a>) in order to produce the final plasmid that was transformed into yeast cells and integrated into the yeast genome. | This sequence was designed to be fully optimized for expression in <i>Saccharomyces cerevisiae</i> and for synthesis. This fragment was then cloned with the bioglue fragment (<a href="https://parts.igem.org/Part:BBa_K5143022">BBa_K5143022</a>) in order to produce the final plasmid that was transformed into yeast cells and integrated into the yeast genome. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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<h1>Sequence and Features</h1> | <h1>Sequence and Features</h1> | ||
<partinfo>BBa_K5143023 SequenceAndFeatures</partinfo> | <partinfo>BBa_K5143023 SequenceAndFeatures</partinfo> | ||
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| + | <h1>References</h1> | ||
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| + | 1. Gilbert, C. et al. Living materials with programmable functionalities grown from engineered microbial co-cultures. Nat. Mater. 20, 691–700 (2021).<br> | ||
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| + | 2. Liljeruhm, J. et al. Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. Journal of Biological Engineering 12, 8 (2018). | ||
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<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
Latest revision as of 14:26, 1 October 2024
Yellow chromoprotein secreted by Saccharomyces cerevisiae and binded to cellulose
Description
Our project aims to create a fully bio-sourced and biodegradable insect trap. To achieve this, the base of our trap will be made of cellulose produced by a bacterium in a co-culture system. The other organism in this co-culture is Saccharomyces cerevisiae, a yeast that will secrete a yellow chromoprotein and a bioglue (BBa_K5143022). The adhesion of the yellow chromoprotein to the cellulose, which forms the base of our trap, will make it coloured, trapping insects that are specifically attracted to yellow.
To allow the secretion and the cellulose binding of the fwYellow, two elements are fused to the fwYellow gene :
1) Upstream of the fwYellow gene : Alpha-factor (BBa_K5143009), a yeast secretion signal allowing the excretion of the fused yellow protein out of the yeast cell.
2) Downstream of the fwYellow gene : Cellulose Binding Domain (BBa_K5143007), allowing the binding of the fused yellow protein to the cellulose.
Because yellow can attract many insects, the chromoprotein could easily be changed by cloning methods in order to change the colour of the trap and attract different targets.
Construction
This sequence was designed to be fully optimized for expression in Saccharomyces cerevisiae and for synthesis. This fragment was then cloned with the bioglue fragment (BBa_K5143022) in order to produce the final plasmid that was transformed into yeast cells and integrated into the yeast genome.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 688
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 688
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 688
Illegal BamHI site found at 1233 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 688
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 688
Illegal NgoMIV site found at 1203
Illegal AgeI site found at 1242 - 1000COMPATIBLE WITH RFC[1000]
References
1. Gilbert, C. et al. Living materials with programmable functionalities grown from engineered microbial co-cultures. Nat. Mater. 20, 691–700 (2021).
2. Liljeruhm, J. et al. Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. Journal of Biological Engineering 12, 8 (2018).