Difference between revisions of "Part:BBa K5034213:Design"
(→‎Design Notes)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
We synthesized the fragment using chemical synthesis method.
+
We synthesized the fragment using a chemical synthesis method, with the aim of expressing the protein in Shewanella and regulating cellular phosphorus metabolism and electron transfer.
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Revision as of 07:11, 29 September 2024


PolyP <->Pi


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We synthesized the fragment using a chemical synthesis method, with the aim of expressing the protein in Shewanella and regulating cellular phosphorus metabolism and electron transfer.

Source

Polyphosphate kinase 1(PPK1) from Citrobacter freundii. NBCI reference sequence: NZ_LR890181.1:c1367205-1365139

References

Wang, X., Wang, X., Hui, K., Wei, W., Zhang, W., Miao, A., . . . Yang, L. (2018). Highly Effective Polyphosphate Synthesis, Phosphate Removal, and Concentration Using Engineered Environmental Bacteria Based on a Simple Solo Medium-Copy Plasmid Strategy. Environmental Science & Technology, 52(1), 214-222. doi:10.1021/acs.est.7b04532