Difference between revisions of "Part:BBa K239001:Design"
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===Design Notes=== | ===Design Notes=== | ||
Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the first BaeR binding site and ends 5 pb after the -10 box. | Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the first BaeR binding site and ends 5 pb after the -10 box. | ||
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===Source=== | ===Source=== |
Latest revision as of 19:24, 21 October 2009
Status: 500 Content-type: text/html
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Status: 500
Content-type: text/html
Software error:
Global symbol "$range" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 250. Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 251, at end of line syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 251, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 261, near "= @_" syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 282, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 286, near "= @_" Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 288. syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 314, near "}" Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.
Design Notes
Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the first BaeR binding site and ends 5 pb after the -10 box.
Source
Genomic sequence from biocyc, Escherichia coli K-12. Native unmodified sequence.
References
Baranova, N., and H. Nikaido (2002) The BaeSR two-component regulatory system activates transcription of the yegMNOB (mdtABCD) transporter gene cluster in Escherichia coli and increases its resistance to novobiocin and deoxycholate. J. Bacteriol. 184:4168–4176.
DiGiuseppe, P. A., and T. J. Silhavy (2003) Signal detection and target gene
induction by the CpxRA two-component system. J. Bacteriol. 185:2432–
2440.
Hagenmaier, S., Stierhof, Y.D., and Henning, U. (1997) A
new periplasmic protein of Escherichia coli which is synthesized
in spheroplasts but not in intact cells. J Bacteriol
179: 2073–2076.
Kunihiko Nishino, Eiji Nikaido, and Akihito Yamaguchi (2007) Regulation of Multidrug Efflux Systems Involved in Multidrug and
Metal Resistance of Salmonella enterica Serovar Typhimurium. JOURNAL OF BACTERIOLOGY, Dec. 2007, p. 9066–9075
Nishino, K., T. Honda, and A. Yamaguchi. (2005) Genome-wide analyses of
Escherichia coli gene expression responsive to the BaeSR two-component
regulatory system. J. Bacteriol. 187:1763–1772.
Price, N. L., and T. L. Raivio. (2009) Characterization of the Cpx Regulon in Escherichia coli Strain MC4100
JOURNAL OF BACTERIOLOGY, Mar. 2009, p. 1798–1815
Raffa, R. G., and T. L. Raivio. (2002) A third envelope stress signal transduction
pathway in Escherichia coli. Mol. Microbiol. 45:1599–1611.
Raivio, T. L., M. W. Laird, J. C. Joly, and T. J. Silhavy. (2000) Tethering of
CpxP to the inner membrane prevents spheroplast induction of the cpx
envelope stress response. Mol. Microbiol. 37:1186–1197.
Snyder, Larry., and Wendy Champness. (2007) Molecular genetics of bacteria, 3rd ed.
ASM Press. Washington, DC.
Wulf, P.D., McGuire, A.M., Liu, X., and Lin, E.C.C. (2002)
Genome-wide profiling of promoter recognition by the twocomponent
response regulator CpxR-P in Escherichia coli.
J Biol Chem 277: 26652–26661.
Yamamoto, K., Ishihama, A. (2005) Transcriptional response of Escherichia
coli to external copper. Mol. Microbiol. 56, 215–227.
Yamamoto K, Ogasawara H, Ishihama A (2008) "Involvement of multiple transcription factors for metal-induced spy gene expression in Escherichia coli." J Biotechnol 133(2):196-200. PMID: 17884222