Difference between revisions of "Part:BBa K268000:Experience"

Revision as of 22:26, 6 September 2012

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Please enter how you used this part and how it worked out.

Applications of BBa_K268000

User Reviews

No review score entered. Earthnut

WARNING: THIS PART STILL CONTAINS AT LEAST ONE FORBIDDEN RESTRICTION SITE (XbaI or PstI) IN THE BACKBONE! ALSO THE PREDICTION ALGORITHM FOR FORBIDDEN SITES DO NOT WORK PROPERLY IN THIS CASE. AT BASEPAIR 46 there is a NotI RESTRICTION SITE OF RFC10.

An analytical digestion with XbaI and PstI was performed to linearize the plasmid, but unexpectedly there was more than one band visible, one at ~3.3&nbbsp;kbp and one at 1.4 kbp.

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UNIQ7df47ba07347af6b-partinfo-00000001-QINU UNIQ7df47ba07347af6b-partinfo-00000002-QINU

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 __NOTOC__
 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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 ===User Reviews===
+{|width='80%' style='border:1px solid gray'
+|-
+|width='10%'|
+<partinfo>BBa_K268000 AddReview number</partinfo>
+<I>Earthnut</I>
+|width='60%' valign='top'|
+<span style="color: red"> WARNING: </span>THIS PART STILL CONTAINS AT LEAST ONE FORBIDDEN RESTRICTION SITE (XbaI or PstI) IN THE BACKBONE! ALSO THE PREDICTION ALGORITHM FOR FORBIDDEN SITES DO NOT WORK PROPERLY IN THIS CASE. AT BASEPAIR 46 there is a NotI RESTRICTION SITE OF RFC10.
+An analytical digestion with XbaI and PstI was performed to linearize the plasmid, but unexpectedly there was more than one band visible, one at ~3.3&nbbsp;kbp and one at 1.4&nbsp;kbp.
+|};
 <!-- DON'T DELETE --><partinfo>BBa_K268000 StartReviews</partinfo>
 <!-- Template for a user review