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<span style="font-size: 150%; font-weight: bold;">Reference documentation</span>
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[1] https://2022.igem.wiki/sjtu-biox-shanghai/design
  
 
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Revision as of 00:58, 29 September 2024


Tyrosinase(BM)

Description: Tyrosinase that converts tyrosine to dopamine and dopamine to dopaquinone.

Gene Circuit Diagram:

Source: Priestia megaterium or Bacillus megaterium

Result:

Construct a brewing yeast vector driven by the GAL1 promoter, using Tyrosinase(BM) as the target gene. By adding an inducer to activate GAL1, the expression of the tyrosinase gene is induced, catalyzing the oxidation of tyrosine into dopamine, which is further oxidized and polymerized to form melanin.Melanin was successfully expressed in yeast.

Reference documentation

[1] https://2022.igem.wiki/sjtu-biox-shanghai/design

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 382
  • 1000
    COMPATIBLE WITH RFC[1000]