Difference between revisions of "Part:BBa K5299200:Design"
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===References=== | ===References=== | ||
− | *[1]: Jaishankar, J., & Srivastava, P. (2020). Strong synthetic stationary phase promoter-based gene expression system for | + | *[1]: Jaishankar, J., & Srivastava, P. (2020). Strong synthetic stationary phase promoter-based gene expression system for |
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Revision as of 15:20, 30 September 2024
P3.1 - BBa_B0030 - BBa_I746916 - BBa_B0015
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Unknown
Design Notes
Created using the Golden Braid method, that utilises type IIS enzymes. We used BsmBI to create Level 0 constructs, and BsaI for Level 1 constructs.
Source
Promoter is BBa_K4583008, originally from [1].
RBS is BBa_B0030.
sfGFP is BBa_I746916, originally from [2].
Terminator is BBa_B0015.
References
- [1]: Jaishankar, J., & Srivastava, P. (2020). Strong synthetic stationary phase promoter-based gene expression system for