Difference between revisions of "Part:BBa K5241001"

Revision as of 06:26, 27 September 2024

Pahdd

Encodes 4-hydroxyphenylpyruvate dioxygenase, involved in melanin synthesis.

Gene Circuit Diagram:

Source: Pseudomonas aeruginosa

Result：Based on Pseudomonas aeruginosa PAO1 [gene=hppD] [protein=4-hydroxyphenylpyruvate dioxygenase], a Pahdd-6xH gene with a 6xH tag is synthesized in pET-21b(+), with the anticipated protein localization within the bacterial cytoplasm

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 56
Illegal EcoRI site found at 227
Illegal EcoRI site found at 701
Illegal PstI site found at 932
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 56
Illegal EcoRI site found at 227
Illegal EcoRI site found at 701
Illegal PstI site found at 932
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 56
Illegal EcoRI site found at 227
Illegal EcoRI site found at 701
Illegal BglII site found at 936
Illegal XhoI site found at 869
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 56
Illegal EcoRI site found at 227
Illegal EcoRI site found at 701
Illegal PstI site found at 932
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 56
Illegal EcoRI site found at 227
Illegal EcoRI site found at 701
Illegal PstI site found at 932
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 Encodes 4-hydroxyphenylpyruvate dioxygenase, involved in melanin synthesis.
+Gene Circuit Diagram:
 <html>
@@ Line 11: / Line 13: @@
 </html>
-Based on Pseudomonas aeruginosa PAO1 [gene=hppD] [protein=4-hydroxyphenylpyruvate dioxygenase], a Pahdd-6xH gene with a 6xH tag is synthesized in pET-21b(+), with the anticipated protein localization within the bacterial cytoplasm
+Source: Pseudomonas aeruginosa
+Result：Based on Pseudomonas aeruginosa PAO1 [gene=hppD] [protein=4-hydroxyphenylpyruvate dioxygenase], a Pahdd-6xH gene with a 6xH tag is synthesized in pET-21b(+), with the anticipated protein localization within the bacterial cytoplasm
 <html>