Difference between revisions of "Part:BBa K5477030"

 
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The XRE-pMEL1-NanoLuc reporter module is a critical component of the AhR (Aryl hydrocarbon receptor) biosensor system, designed to detect and report the presence of environmental toxins, such as polycyclic aromatic hydrocarbons (PAHs), dioxins and polychlorinated biphenyls (PCBs). In this module, the XRE (Xenobiotic Response Element) is the DNA sequence that AhR, once activated by ligand binding, specifically recognizes and binds to. The pMEL1 promoter, which is activated upon AhR binding to the XRE sequence, drives the expression of the NanoLuc reporter gene. NanoLuc, a highly sensitive and small luciferase enzyme, produces a bioluminescent signal under presence of contaminants.
 
The XRE-pMEL1-NanoLuc reporter module is a critical component of the AhR (Aryl hydrocarbon receptor) biosensor system, designed to detect and report the presence of environmental toxins, such as polycyclic aromatic hydrocarbons (PAHs), dioxins and polychlorinated biphenyls (PCBs). In this module, the XRE (Xenobiotic Response Element) is the DNA sequence that AhR, once activated by ligand binding, specifically recognizes and binds to. The pMEL1 promoter, which is activated upon AhR binding to the XRE sequence, drives the expression of the NanoLuc reporter gene. NanoLuc, a highly sensitive and small luciferase enzyme, produces a bioluminescent signal under presence of contaminants.
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The pUUS plasmid is a multi-copy plasmid used in yeast, carrying a URA3 marker for selection in uracil auxotrophic strains. In our system, pUUS is utilized in the reporter module, enabling the expression of the reporter gene, NanoLuc [https://parts.igem.org/Part:BBa_K1680009| BBa_K1680009]
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Revision as of 20:27, 26 September 2024


XRE-pMEL1-NanoLuc reporter module

The XRE-pMEL1-NanoLuc reporter module is a critical component of the AhR (Aryl hydrocarbon receptor) biosensor system, designed to detect and report the presence of environmental toxins, such as polycyclic aromatic hydrocarbons (PAHs), dioxins and polychlorinated biphenyls (PCBs). In this module, the XRE (Xenobiotic Response Element) is the DNA sequence that AhR, once activated by ligand binding, specifically recognizes and binds to. The pMEL1 promoter, which is activated upon AhR binding to the XRE sequence, drives the expression of the NanoLuc reporter gene. NanoLuc, a highly sensitive and small luciferase enzyme, produces a bioluminescent signal under presence of contaminants.

The pUUS plasmid is a multi-copy plasmid used in yeast, carrying a URA3 marker for selection in uracil auxotrophic strains. In our system, pUUS is utilized in the reporter module, enabling the expression of the reporter gene, NanoLuc BBa_K1680009


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 59
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 59
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 59
    Illegal BamHI site found at 3
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 59
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 59
  • 1000
    COMPATIBLE WITH RFC[1000]