Difference between revisions of "Part:BBa K5492010"
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| − | <span class='h3bb'> | + | <span class='h3bb'>Experiments</span> |
| + | ==PCR== | ||
| + | We have found the following PCR protocol the most optimal for the part: | ||
| + | https://static.igem.wiki/teams/5492/registry/dao-pcr-plan.png | ||
| + | |||
| + | Optimised dao: | ||
| + | |||
| + | We added the following substrates in order: | ||
| + | |||
| + | https://static.igem.wiki/teams/5492/registry/dao-pcr-substr.png | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | We added the 6X DNA Loading dye to each PCR tubes. | ||
| + | We loaded the wells of the gels with 12μL of DAO solution in the following order: | ||
| + | 1. D31O | ||
| + | 2. D32O | ||
| + | 3. D33O | ||
| + | 4. D34O | ||
| + | 5. Ladder | ||
| + | |||
| + | |||
| + | |||
| + | https://static.igem.wiki/teams/5492/registry/opt-dao-pcr.png | ||
| + | |||
<partinfo>BBa_K5492010 SequenceAndFeatures</partinfo> | <partinfo>BBa_K5492010 SequenceAndFeatures</partinfo> | ||
Revision as of 14:27, 1 October 2024
Twist adapter forward primer
Forward primer sequence of the adapter sequence utilised by Twist Bioscience. Utilised in PCR reactions for part BBa_K5492202 and BBa_K5492203.
Experiments
PCR
We have found the following PCR protocol the most optimal for the part:
Optimised dao:
We added the following substrates in order:
We added the 6X DNA Loading dye to each PCR tubes.
We loaded the wells of the gels with 12μL of DAO solution in the following order:
1. D31O
2. D32O
3. D33O
4. D34O
5. Ladder
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]