Difference between revisions of "Part:BBa K5492403:Design"
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===Source=== | ===Source=== | ||
| − | https://www.ncbi.nlm.nih.gov/nuccore/AH003685, [https://parts.igem.org/Part:BBa_K5492401 BBa_K5492401], | + | https://www.ncbi.nlm.nih.gov/nuccore/AH003685, [https://parts.igem.org/Part:BBa_K5492401 BBa_K5492401], |
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===References=== | ===References=== | ||
https://www.genscript.com/gentitan-gene-fragments.html | https://www.genscript.com/gentitan-gene-fragments.html | ||
Revision as of 06:51, 2 October 2024
Optimised HNMT - pETDUET-1
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 129
Illegal EcoRI site found at 235
Illegal XbaI site found at 47 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 129
Illegal EcoRI site found at 235 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 129
Illegal EcoRI site found at 235
Illegal BglII site found at 1015
Illegal BamHI site found at 123
Illegal XhoI site found at 1064 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 129
Illegal EcoRI site found at 235
Illegal XbaI site found at 47 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 129
Illegal EcoRI site found at 235
Illegal XbaI site found at 47
Illegal NgoMIV site found at 1034 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Restriction sites adapted to pETDUET-1, with adjustions also added for proper framing of the gene.
Source
https://www.ncbi.nlm.nih.gov/nuccore/AH003685, BBa_K5492401,