Difference between revisions of "Part:BBa K5477012"

Revision as of 15:28, 29 September 2024

NCOA - Nuclear receptor coactivator (Steroid Receptor Coactivator)

Nuclear receptor coactivator (NCOA), also known as SRC (Steroid Receptor Coactivator), is a member of a family of transcriptional coactivators that enhance the activity of nuclear receptors. NCOA plays a pivotal role in regulating gene expression by acting as a bridge between nuclear receptors, such as the Aryl hydrocarbon receptor (AhR). When nuclear receptors are activated by their ligands, NCOA is recruited to the receptor complex, where it interacts with histone acetyltransferases (HATs) and other chromatin-modifying enzymes to promote an open chromatin structure, facilitating transcriptional activation (3) (4).

In our receptor module, NCOA functions as a coactivator for nuclear receptors like AhR BBa_K3793004, enhancing their ability to regulate the expression of detoxification genes when activated by ligands such as polycyclic aromatic hydrocarbons (PAHs) or polychlorinated biphenyls (PCBs) (1) (2). This facilitates transcriptional efficiency of AhR upon ligand binding initiating the transcription of NanoLuc in our reporter module | BBa_K5477030. This is also a part of our following composite and devices: | BBa_K5477026, | BBa_K5477041 and | BBa_K5477042.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1079
Illegal EcoRI site found at 1701
Illegal SpeI site found at 528
Illegal SpeI site found at 829
Illegal SpeI site found at 1896
Illegal PstI site found at 588
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1079
Illegal EcoRI site found at 1701
Illegal NheI site found at 2841
Illegal SpeI site found at 528
Illegal SpeI site found at 829
Illegal SpeI site found at 1896
Illegal PstI site found at 588
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1079
Illegal EcoRI site found at 1701
Illegal BamHI site found at 2343
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1079
Illegal EcoRI site found at 1701
Illegal SpeI site found at 528
Illegal SpeI site found at 829
Illegal SpeI site found at 1896
Illegal PstI site found at 588
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1079
Illegal EcoRI site found at 1701
Illegal SpeI site found at 528
Illegal SpeI site found at 829
Illegal SpeI site found at 1896
Illegal PstI site found at 588
1000
COMPATIBLE WITH RFC[1000]

References

1. Carambia, A., Schuran, F.A. The aryl hydrocarbon receptor in liver inflammation. Semin Immunopathol 43, 563–575 (2021). https://doi.org/10.1007/s00281-021-00867-8

2. Goedtke L, Sprenger H, Hofmann U, Schmidt FF, Hammer HS, Zanger UM, Poetz O, Seidel A, Braeuning A, Hessel-Pras S. Polycyclic Aromatic Hydrocarbons Activate the Aryl Hydrocarbon Receptor and the Constitutive Androstane Receptor to Regulate Xenobiotic Metabolism in Human Liver Cells. Int J Mol Sci. 2020 Dec 31;22(1):372. doi: 10.3390/ijms22010372. PMID: 33396476; PMCID: PMC7796163.

3. Onate SA, Boonyaratanakornkit V, Spencer TE, et al. The steroid receptor coactivator-1 contains multiple receptor interacting and activation domains that cooperatively enhance the activation function 1 (AF1) and AF2 domains of steroid receptors. J Biol Chem. 1998;273(20):12101-12108. doi:10.1074/jbc.273.20.12101

4. Oñate SA, Tsai SY, Tsai MJ, O'Malley BW. Sequence and characterization of a coactivator for the steroid hormone receptor superfamily. Science. 1995;270(5240):1354-1357. doi:10.1126/science.270.5240.1354

@@ Line 5: / Line 5: @@
 Nuclear receptor coactivator (NCOA), also known as SRC (Steroid Receptor Coactivator), is a member of a family of transcriptional coactivators that enhance the activity of nuclear receptors. NCOA plays a pivotal role in regulating gene expression by acting as a bridge between nuclear receptors, such as the Aryl hydrocarbon receptor (AhR). When nuclear receptors are activated by their ligands, NCOA is recruited to the receptor complex, where it interacts with histone acetyltransferases (HATs) and other chromatin-modifying enzymes to promote an open chromatin structure, facilitating transcriptional activation (3) (4).
-In our receptor module, NCOA functions as a coactivator for nuclear receptors like AhR, enhancing their ability to regulate the expression of detoxification genes when activated by ligands such as polycyclic aromatic hydrocarbons (PAHs) or polychlorinated biphenyls (PCBs) (1) (2). This facilitates transcriptional efficiency of AhR upon ligand binding initiating the transcription of NanoLuc in our reporter module.
+In our receptor module, NCOA functions as a coactivator for nuclear receptors like AhR [https://parts.igem.org/Part:BBa_K3793004| BBa_K3793004], enhancing their ability to regulate the expression of detoxification genes when activated by ligands such as polycyclic aromatic hydrocarbons (PAHs) or polychlorinated biphenyls (PCBs) (1) (2). This facilitates transcriptional efficiency of AhR upon ligand binding initiating the transcription of NanoLuc in our reporter module [https://parts.igem.org/Part:BBa_K5477030 | BBa_K5477030]. This is also a part of our following composite and devices:  [https://parts.igem.org/Part:BBa_K5477026 | BBa_K5477026],  [https://parts.igem.org/Part:BBa_K5477041 | BBa_K5477041] and [https://parts.igem.org/Part:BBa_K5477042 | BBa_K5477042].
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