Difference between revisions of "Part:BBa K5246048"
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===Introduction===
 
===Introduction===
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Vilnius-Lithuania iGEM 2024 project <HTML><b><a href="https://2024.igem.wiki/vilnius-lithuania" target="_blank">Synhesion</a></b></html> aspires to create biodegradable and environmentally friendly adhesives. We were inspired by bacteria, which naturally produce adhesives made from polysaccharides. Two bacteria from aquatic environments - <I> C. crescentus </I> and <I> H. baltica </I> - harness 12 protein synthesis pathways to produce sugars, anchoring them to the surfaces. We aimed to transfer the polysaccharide synthesis pathway to industrially used <I>E. coli</I> bacteria to produce adhesives. Our team concomitantly focused on creating a novel <I>E. coli</I> strain for more efficient production of adhesives.
  
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===Usage and Biology===
 
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TBA
 
  
 
===Sequence and Features===
 
===Sequence and Features===
 
<partinfo>BBa_K5246048 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5246048 SequenceAndFeatures</partinfo>
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===Functional Parameters===
 
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<partinfo>BBa_K5246048 parameters</partinfo>
 
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===Experimental characterization===
 
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===References===
 

Revision as of 21:45, 1 October 2024


H.Baltica hfsJ-hfsL-hfsK Part of the polysaccharide tetrad assembly system

Introduction

Vilnius-Lithuania iGEM 2024 project Synhesion aspires to create biodegradable and environmentally friendly adhesives. We were inspired by bacteria, which naturally produce adhesives made from polysaccharides. Two bacteria from aquatic environments - C. crescentus and H. baltica - harness 12 protein synthesis pathways to produce sugars, anchoring them to the surfaces. We aimed to transfer the polysaccharide synthesis pathway to industrially used E. coli bacteria to produce adhesives. Our team concomitantly focused on creating a novel E. coli strain for more efficient production of adhesives.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 274
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 274
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 708
    Illegal BglII site found at 2877
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 274
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 274
    Illegal NgoMIV site found at 1029
    Illegal AgeI site found at 2927
  • 1000
    COMPATIBLE WITH RFC[1000]