Difference between revisions of "Part:BBa K5477009"

Revision as of 14:23, 29 September 2024

Lex6Op - six tandem copies of LexA operator

The Lex6Op refers to six tandem copies of the LexA operator. These operator sequences serve as binding sites for transcription factors that contain a LexA DNA-binding domain. This creates a platform for transcriptional activation of the reporter system upon presence of the ligand (1) (2).

In our reporter module | BBa_K5477031 , the Lex6Op is positioned upstream of the promoter (pLEU2), meaning the LexA fused with either the wildtype or mutant ERα will bind to these operators that drive the expression of our reporter gene NanoLuc. The devices that contain this part are: | BBa_K5477043, | BBa_K5477044, | BBa_K5477045 and | BBa_K5477046

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

1. Shaw, W.M., et al., Engineering a Model Cell for Rational Tuning of GPCR Signaling. Cell, 2019. 177(3): p. 782-796 e27.

2. Zhou, T., Liang, Z. & Marchisio, M.A. Engineering a two-gene system to operate as a highly sensitive biosensor or a sharp switch upon induction with β-estradiol. Sci Rep 12, 21791 (2022). https://doi.org/10.1038/s41598-022-26195-x

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K5477009 short</partinfo>
-The Lex6Op refers to six tandem copies of the LexA operator. These operator sequences serve as binding sites for transcription factors that contain a LexA DNA-binding domain. This creates a platform for transcriptional activation of the reporter system upon presence of the ligand (1) (2).
+The <i>Lex6Op</i> refers to six tandem copies of the LexA operator. These operator sequences serve as binding sites for transcription factors that contain a LexA DNA-binding domain. This creates a platform for transcriptional activation of the reporter system upon presence of the ligand (1) (2).
-In our reporter module, the Lex6Op is positioned upstream of the promoter (pLEU2), meaning the LexA fused with either the wildtype or mutant ERα or ERRγ will bind to these operators that drive the expression of our reporter gene NanoLuc.
+In our reporter module [https://parts.igem.org/Part:BBa_K5477031 | BBa_K5477031] , the <i>Lex6Op</i> is positioned upstream of the promoter (pLEU2), meaning the LexA fused with either the wildtype or mutant ERα will bind to these operators that drive the expression of our reporter gene <i>NanoLuc</i>. The devices that contain this part are: [https://parts.igem.org/Part:BBa_K5477043 | BBa_K5477043], [https://parts.igem.org/Part:BBa_K5477044 | BBa_K5477044], [https://parts.igem.org/Part:BBa_K5477045 | BBa_K5477045] and [https://parts.igem.org/Part:BBa_K5477046 | BBa_K5477046]