Difference between revisions of "Part:BBa K5477008"
Line 3: Line 3:
 
<partinfo>BBa_K5477008 short</partinfo>
 
<partinfo>BBa_K5477008 short</partinfo>
  
−
XRE is Xenobiotic Response Element found in eukaryotic cell upstream of inducible target genes for the transcription factor aryl hydrocarbon receptor (AhR) which is responsible for signal transduction of pollutants (1) (2). XRE was used in our reporter module to drive the expression of NanoLuc under the presence of PAHs or PCB.  This reporter module is combined with the AhR receptor module and therefore comprises our biosensor system to detect PAHs and PCBs.
+
XRE is Xenobiotic Response Element found in eukaryotic cell upstream of inducible target genes for the transcription factor aryl hydrocarbon receptor (AhR) [https://parts.igem.org/Part:BBa_K3793004| BBa_K3793004]  which is responsible for signal transduction of pollutants (1) (2). XRE was used in our reporter module to drive the expression of NanoLuc under the presence of PAHs or PCB [https://parts.igem.org/Part:BBa_K5477030| BBa_K5477030].  This reporter module is combined with the AhR receptor module and therefore comprises our biosensor systems to detect PAHs and PCBs [https://parts.igem.org/Part:BBa_K5477041| BBa_K5477041] and [https://parts.igem.org/Part:BBa_K5477042| BBa_K5477042] .
  
  

Revision as of 14:17, 29 September 2024


XRE - Xenobiotic Response Element

XRE is Xenobiotic Response Element found in eukaryotic cell upstream of inducible target genes for the transcription factor aryl hydrocarbon receptor (AhR) BBa_K3793004 which is responsible for signal transduction of pollutants (1) (2). XRE was used in our reporter module to drive the expression of NanoLuc under the presence of PAHs or PCB BBa_K5477030. This reporter module is combined with the AhR receptor module and therefore comprises our biosensor systems to detect PAHs and PCBs BBa_K5477041 and BBa_K5477042 .


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 59
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 59
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 59
    Illegal BamHI site found at 3
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 59
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 59
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Huang G, Elferink CJ. A novel nonconsensus xenobiotic response element capable of mediating aryl hydrocarbon receptor-dependent gene expression. Mol Pharmacol. 2012 Mar;81(3):338-47. doi: 10.1124/mol.111.075952. Epub 2011 Nov 23. PMID: 22113079; PMCID: PMC3286297.

2. Kuramoto, Nobuyuki & Baba, Katsuhiro & Gion, Keiko & Sugiyama, Chie & Taniura, Hideo & Yoneda, Yukio. (2003). Xenobiotic response element binding enriched in both nuclear and microsomal fractions of rat cerebellum. Journal of neurochemistry. 85. 264-73. 10.1046/j.1471-4159.2003.01679.x.