Difference between revisions of "Part:BBa K5152008"
| Line 8: | Line 8: | ||
However, because we ordered the wrong DNA, we didn't have time to clone this construct, and no experiments were conducted on it. | However, because we ordered the wrong DNA, we didn't have time to clone this construct, and no experiments were conducted on it. | ||
| − | + | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 07:04, 26 September 2024
MerR-pMerT mercury sensing chromoprotein reporter device
This device contains the mercury-inducible promoter pMerT and co-expresses its repressor protein MerR, which specifically binds to mercury. When mercury is present, the pMerT promoter drives the expression of the chromoprotein tsPurple, serving as the reporter signal. The design was inspired by iGEM 2010 Team Peking (BBa_K346002).
We used tsPurple as the reporter gene, a purple chromoprotein chosen for its visible color. This helps create a user-friendly and cost-effective mercury biosensor without needing expensive equipment or special techniques.
However, because we ordered the wrong DNA, we didn't have time to clone this construct, and no experiments were conducted on it.
Usage and Biology
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 35
Illegal NheI site found at 58 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]