Difference between revisions of "Part:BBa K5152005"

Revision as of 00:02, 27 September 2024

pCadA cadmium sensing chromoprotein reporter device

This part was used to create a cadmium-sensing biosensor for our project. The design of this part was inspired by iGEM 2009 Team Newcastle (BBa_K174015).

Our goal is to develop heavy metal biosensors that don't require special techniques or expensive equipment, so we used chromoproteins for their visible colours.

We designed this part and ordered it from IDT gBlock. However, we consistently failed to obtain successful transformants for unknown reasons. Despite using the same assembly process as other constructs, which succeeded, this one did not. The cause remains unclear.

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 228
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 This part was used to create a cadmium-sensing biosensor for our project. The design of this part was inspired by iGEM 2009 Team Newcastle (<partinfo>BBa_K174015</partinfo>).
-Our goal is to develop heavy metal biosensors that don't require special techniques or expensive equipment, so we used chromoproteins for their visible colors.
+Our goal is to develop heavy metal biosensors that don't require special techniques or expensive equipment, so we used chromoproteins for their visible colours.
 We designed this part and ordered it from IDT gBlock. However, we consistently failed to obtain successful transformants for unknown reasons. Despite using the same assembly process as other constructs, which succeeded, this one did not. The cause remains unclear.