Difference between revisions of "Part:BBa K5492403"

Revision as of 12:14, 1 October 2024

Optimised HNMT - pETDUET-1

Optimised coding sequence of human Histamine-N-Methyltransferase appropiated for insertion into pETDUET-1. This part uses BBa_K5492100 designed for the conservation of sequences during synthesis and PCR reactions. Adapter sequences from GenScript are added to the 5' and 3' end of the part. The primers for this sequence can be found here: BBa_K5492020 BBa_K5492021

Usage and Biology

Histamine-N-Methlytransferase (HNMT) takes part in the inactivation of histamine. It transfers a methyl group to histamine, and the resulting N-methyl histamine isn’t able to bind to histamine receptors, thus reducing the biological effect of the compound. Limiting the level of histamine effect can be an appropriate approach in cases of inflammation, where you can anticipate the histamine level increase. On our skin this includes some chronic diseases such as psoriasis, but more often would be useful in handling the acute increase of histamine level, such as a wasp bite. The decrease in oral histamine administration may also help to prevent some acute GI inflammation and smoothing the symptoms of some chronic bowel diseases.