Difference between revisions of "Part:BBa K5477003"
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pMEL1 encodes for secreted alpha-galactosidase is an enzyme required for the catabolic breakdown of melibiose into glucose and galactose. This process is regulated by several GAL genes. The enzyme plays a crucial role in carbohydrate metabolism, facilitating the utilization of melibiose as a carbon source (1) (2) (3).
 
pMEL1 encodes for secreted alpha-galactosidase is an enzyme required for the catabolic breakdown of melibiose into glucose and galactose. This process is regulated by several GAL genes. The enzyme plays a crucial role in carbohydrate metabolism, facilitating the utilization of melibiose as a carbon source (1) (2) (3).
  
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In our system, pMEL1 was used to drive the expression of our reporter module with NanoLuc. This reporter module is combined with the AhR receptor module and therefore comprises our biosensor system.
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In our system, pMEL1 was used to drive the expression of our reporter module with NanoLuc [https://parts.igem.org/Part:BBa_K5477030 | BBa_K5477030]. This reporter module is combined with the AhR receptor module and therefore comprises our biosensor systems [https://parts.igem.org/Part:BBa_K5477041 | BBa_K5477041] and [https://parts.igem.org/Part:BBa_K5477042 | BBa_K5477042] .
  
  

Revision as of 13:40, 29 September 2024


pMEL1 - constitutive core promoter in Saccharomyces cerevisiae

pMEL1 encodes for secreted alpha-galactosidase is an enzyme required for the catabolic breakdown of melibiose into glucose and galactose. This process is regulated by several GAL genes. The enzyme plays a crucial role in carbohydrate metabolism, facilitating the utilization of melibiose as a carbon source (1) (2) (3).

In our system, pMEL1 was used to drive the expression of our reporter module with NanoLuc | BBa_K5477030. This reporter module is combined with the AhR receptor module and therefore comprises our biosensor systems | BBa_K5477041 and | BBa_K5477042 .


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Mortimer RK, Contopoulou CR, King JS. Genetic and physical maps of Saccharomyces cerevisiae, Edition 11. Yeast. 1992 Oct;8(10):817-902. doi: 10.1002/yea.320081002. PMID: 1413997.

2. Naumov G, Turakainen H, Naumova E, Aho S, Korhola M. A new family of polymorphic genes in Saccharomyces cerevisiae: alpha-galactosidase genes MEL1-MEL7. Mol Gen Genet. 1990 Oct;224(1):119-28. doi: 10.1007/BF00259458. PMID: 1980522.

3. Sumner-Smith M, Bozzato RP, Skipper N, Davies RW, Hopper JE. Analysis of the inducible MEL1 gene of Saccharomyces carlsbergensis and its secreted product, alpha-galactosidase (melibiase). Gene. 1985;36(3):333-40. doi: 10.1016/0378-1119(85)90188-x. PMID: 3000884.