Difference between revisions of "Part:BBa K5246069"
Line 4: Line 4:
  
 
===Introduction===
 
===Introduction===
 +
Vilnius-Lithuania iGEM 2024 project <HTML><b><a href="https://2024.igem.wiki/vilnius-lithuania" target="_blank">Synhesion</a></b></html> aspires to create biodegradable and environmentally friendly adhesives. We were inspired by bacteria, which naturally produce adhesives made from polysaccharides. Two bacteria from aquatic environments - <I> C. crescentus </I> and <I> H. baltica </I> - harness 12 protein synthesis pathways to produce sugars, anchoring them to the surfaces. We aimed to transfer the polysaccharide synthesis pathway to industrially used <I>E. coli</I> bacteria to produce adhesives. Our team concomitantly focused on creating a novel <I>E. coli</I> strain for more efficient production of adhesives.
  
 +
See Vilnius-Lithuania iGEM 2024 project <HTML><b><a href="https://2024.igem.wiki/vilnius-lithuania" target="_blank">Synhesion</a></b></html> for more information.
  
−
===Usage and Biology===
+
===Biology and usage===
−
TBA
+
  
−
===Sequence and Features===
 
−
<partinfo>BBa_K5246069 SequenceAndFeatures</partinfo>
 
  
 +
Cas9 gRNA targeting <i>wecF</i>.
  
−
===Functional Parameters===
+
===Sequence and Features===
−
<partinfo>BBa_K5246069 parameters</partinfo>
+
<partinfo>BBa_K5246069 SequenceAndFeatures</partinfo>
  
−
===Experimental characterization===
 
  
  
 
===References===
 
===References===

Revision as of 20:28, 1 October 2024


gRNA for wecF gene editing with CRISPR/Cas9

Introduction

Vilnius-Lithuania iGEM 2024 project Synhesion aspires to create biodegradable and environmentally friendly adhesives. We were inspired by bacteria, which naturally produce adhesives made from polysaccharides. Two bacteria from aquatic environments - C. crescentus and H. baltica - harness 12 protein synthesis pathways to produce sugars, anchoring them to the surfaces. We aimed to transfer the polysaccharide synthesis pathway to industrially used E. coli bacteria to produce adhesives. Our team concomitantly focused on creating a novel E. coli strain for more efficient production of adhesives.

See Vilnius-Lithuania iGEM 2024 project Synhesion for more information.

Biology and usage

Cas9 gRNA targeting wecF.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References