Difference between revisions of "Part:BBa K5477000"

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===References===
 
===References===
  
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1. Barlowe, C., and Miller, E. A. (2013). **Secretory protein biogenesis and traffic in the early secretory pathway.** *Genetics*, 193(2), 383-410. https://doi.org/10.1534/genetics.112.143826
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1. Cosson P, Démollière C, Hennecke S, Duden R, Letourneur F. **Delta- and zeta-COP, two coatomer subunits homologous to clathrin-associated proteins, are involved in ER retrieval.** *EMBO J.* 1996 Apr 15;15(8):1792-8. PMID: 8617224; PMCID: PMC450095.
  
 
2. Lee, M. E., DeLoache, W. C., Cervantes, B., & Dueber, J. E. (2015). **A highly characterized yeast toolkit for modular, multipart assembly.** *ACS Synthetic Biology*, 4(9), 975-986. https://doi.org/10.1021/sb500366v
 
2. Lee, M. E., DeLoache, W. C., Cervantes, B., & Dueber, J. E. (2015). **A highly characterized yeast toolkit for modular, multipart assembly.** *ACS Synthetic Biology*, 4(9), 975-986. https://doi.org/10.1021/sb500366v
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1 Cosson P, Démollière C, Hennecke S, Duden R, Letourneur F. Delta- and zeta-COP, two coatomer subunits homologous to clathrin-associated proteins, are involved in ER retrieval. EMBO J. 1996 Apr 15;15(8):1792-8. PMID: 8617224; PMCID: PMC450095.
 
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2 Lee, Michael & DeLoache, William & Cervantes, Bernardo & Dueber, John. (2015). A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS synthetic biology. 4. 10.1021/sb500366v.
 

Revision as of 18:26, 25 September 2024

pRET2 - medium strong constitutive promoter in Saccharomyces cerevisiae

RET2 is the delta subunit of the coatomer complex (COPI), which coats transport vesicles derived from the Golgi apparatus. It plays a key role in retrograde transport between the Golgi and endoplasmic reticulum (ER), ensuring proper protein and membrane trafficking within the cell (1).

Usage and Biology

From a paper of Lee et al. 2015, Figure 3A shows the relative strengths of 19 constitutive promoters by measuring fluorescence from two reporters, mRuby2 and Venus. From the data, pRET2 appears to be positioned in the medium-to-lower range of constitutive promoter strengths.

600px


In our system, pRET2 is used to drive the expression of our receptor modules – Aryl hydrocarbon receptor - AhR (particularly Aryl hydrocarbon receptor nuclear translocator - ARNT and Nuclear Receptor Coactivator- NCOA), LexA domain fused with ligand-binding domain of Estrogen Receptor alpha LexA-ERα, with Estrogen-related Receptor gamma ligand-binding domain LexA-ERRγ and the mutant ligand-binding domain of ERα LexA-mERα.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 182
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 37
  • 1000
    COMPATIBLE WITH RFC[1000]

References

1. Cosson P, Démollière C, Hennecke S, Duden R, Letourneur F. **Delta- and zeta-COP, two coatomer subunits homologous to clathrin-associated proteins, are involved in ER retrieval.** *EMBO J.* 1996 Apr 15;15(8):1792-8. PMID: 8617224; PMCID: PMC450095.

2. Lee, M. E., DeLoache, W. C., Cervantes, B., & Dueber, J. E. (2015). **A highly characterized yeast toolkit for modular, multipart assembly.** *ACS Synthetic Biology*, 4(9), 975-986. https://doi.org/10.1021/sb500366v