Difference between revisions of "Part:BBa K5036045"
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lang=EN style='font-size:11.0pt;line-height:115%'>this figure illustrates the structure of MCP-MMP9 Nanobody which is attached to our switch | lang=EN style='font-size:11.0pt;line-height:115%'>this figure illustrates the structure of MCP-MMP9 Nanobody which is attached to our switch | ||
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We measured the effect of the nanobodies on the MCP-MS2 binding stability, so we measured MCP-MS2 before and after binding to nanobody1 | We measured the effect of the nanobodies on the MCP-MS2 binding stability, so we measured MCP-MS2 before and after binding to nanobody1 | ||
Revision as of 17:05, 25 September 2024
MCP-MMP9 Nanobody1
Part Description
Our engineered switch contains a nanobody that recognizes MMP9, an enzyme elevated in injured cells. This nanobody is linked to MCP protein, which is attached to MS2 from the other end because of its high affinity for MS2.
Usage
Our TID device contains MCP protein linked to specific sensors (nanobodies) that detect MMP9, a protein elevated within cells after tissue injury. TID switches from off state to on state when MMP9 is present in the cell cytoplasm. This triggers the circularization of YAP mRNA, which is essential for protein production
this figure illustrates the structure of MCP-MMP9 Nanobody which is attached to our switch .
Dry lab Characterization
We measured the effect of the nanobodies on the MCP-MS2 binding stability, so we measured MCP-MS2 before and after binding to nanobody1
MCP-MS2
Alignment Plot
3D structure of MCP binded to MS2-Aptamers
this figure show That the alignment plot scores high diagonal intensity which indicates the similarity between our structures and the experimental one .
MS2-MCP-NB1
Alignment Plot
3D structure of MCP binded to MS2-Aptamers
this figure show That The alignment plot scores higher diagonal intensity than MCB-MS2 plot which indicates higher protein stability in the presence of NB1 .
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]