Difference between revisions of "Part:BBa K5115010"

Revision as of 14:52, 24 September 2024

hypF

It's used to build up the hydrogenase which ruduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system BBa_K5115019(ribozyme+RBS+hypF+stem-loop). And then it will eventually make up parts BBa_K5115066(mineral, U module)and BBa_K5115067(mineral, F module), which are used for further experiments.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 424
Illegal XhoI site found at 2263
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 29
Illegal NgoMIV site found at 250
Illegal NgoMIV site found at 274
Illegal NgoMIV site found at 635
Illegal NgoMIV site found at 2035
Illegal NgoMIV site found at 2435
1000
COMPATIBLE WITH RFC[1000]

Revision as of 14:51, 24 September 2024 (view source) Chenliyue (Talk \| contribs) ← Older edit		Revision as of 14:52, 24 September 2024 (view source) Chenliyue (Talk \| contribs) Newer edit →
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	===Usage and Biology===		===Usage and Biology===
−	It's used to build up the hydrogenase which ruduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system [https://parts.igem.org/Part:BBa_K5115019 BBa_K5115019(ribozyme+RBS+hypF+stem-loop)]. And then it will eventually make up parts [https://parts.igem.org/Part:BBa_K5115066 BBa_K5115066(mineral, U module)] and [https://parts.igem.org/Part:BBa_K5115067 BBa_K5115067(mineral, F module)], which are used for further experiments.	+	It's used to build up the hydrogenase which ruduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system [https://parts.igem.org/Part:BBa_K5115019 BBa_K5115019(ribozyme+RBS+hypF+stem-loop)]. And then it will eventually make up parts [https://parts.igem.org/Part:BBa_K5115066 BBa_K5115066(mineral, U module)]and [https://parts.igem.org/Part:BBa_K5115067 BBa_K5115067(mineral, F module)], which are used for further experiments.