Difference between revisions of "Part:BBa K5115006"
Line 9: Line 9:
  
 
===Usage and Biology===
 
===Usage and Biology===
−
It's used to buid up the hydrogenase which ruduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system [https://parts.igem.org/Part:BBa_K5115015 BBa_K5115015(ribozyme+RBS+hoxH+stem-loop)]. And then it will eventually make up parts like [https://parts.igem.org/Part:BBa_K5115066 BBa_K5115066(mineral, U module)], which are used for further experiments
+
It's used to buid up the hydrogenase which ruduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system [https://parts.igem.org/Part:BBa_K5115015 BBa_K5115015(ribozyme+RBS+hoxW+stem-loop)]. And then it will eventually make up parts like [https://parts.igem.org/Part:BBa_K5115066 BBa_K5115066(mineral, U module)], which are used for further experiments.
  
  

Revision as of 14:12, 24 September 2024


hoxW

contributed by Fudan iGEM 2023

Introduction

The hoxW is a subunit of the hox and hyp operonBBa_K5115020.

Usage and Biology

It's used to buid up the hydrogenase which ruduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system BBa_K5115015(ribozyme+RBS+hoxW+stem-loop). And then it will eventually make up parts like BBa_K5115066(mineral, U module), which are used for further experiments.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 184
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References