Difference between revisions of "Part:BBa K5115003"

Revision as of 15:18, 24 September 2024

hoxU, without stop codon

It's used to build up the hydrogenase which reduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system BBa_K5115012(ribozyme+RBS+hoxU+stem-loop). And then it will eventually make up parts BBa_K5115066(mineral, U module)and BBa_K5115067(mineral, F module), which are used for further experiment.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 410
Illegal BglII site found at 488
Illegal XhoI site found at 418
Illegal XhoI site found at 610
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 371
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 192
Illegal BsaI site found at 354
Illegal SapI.rc site found at 303

@@ Line 9: / Line 9: @@
 ===Usage and Biology===
-It's used to buid up the hydrogenase which ruduces the nickel ion. In our experiment, it was construct into our ribozyme-assisted polycistronic co-expression system [https://parts.igem.org/Part:BBa_K5115012 BBa_K5115012]
+It's used to build up the hydrogenase which reduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system [https://parts.igem.org/Part:BBa_K5115012 BBa_K5115012(ribozyme+RBS+hoxU+stem-loop)]. And then it will eventually make up parts [https://parts.igem.org/Part:BBa_K5115066 BBa_K5115066(mineral, U module)]and [https://parts.igem.org/Part:BBa_K5115067 BBa_K5115067(mineral, F module)], which are used for further experiment.
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