Difference between revisions of "Part:BBa K5490006"
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| + | Meško M, Lebar T, Dekleva P, Jerala R, Benčina M. Engineering and Rewiring of a Calcium-Dependent Signaling Pathway. ACS Synth Biol. 2020 Aug 21;9(8):2055-2065. doi: 10.1021/acssynbio.0c00133. Epub 2020 Jul 20. PMID: 32643923; PMCID: PMC7467823. | ||
Latest revision as of 23:59, 28 September 2024
mNFAT
murine NFAT regulatory domain In its natural state, NFAT contains two Nuclear Localization Signals (NLS) that enable nuclear translocation. However, in this engineered version, one NLS has been removed, leaving only a single NLS to reduce background transcription. The NFAT domain is activated through phosphorylation by calcineurin in response to calcium signaling. This domain is critical for calcium-dependent activation in cellular processes, as the increase in cytosolic calcium activates calmodulin, which, in turn, activates calcineurin.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1116
Illegal PstI site found at 934
Illegal PstI site found at 1188 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1116
Illegal NheI site found at 505
Illegal PstI site found at 934
Illegal PstI site found at 1188 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1116
Illegal BamHI site found at 983
Illegal XhoI site found at 1222 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1116
Illegal PstI site found at 934
Illegal PstI site found at 1188 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1116
Illegal PstI site found at 934
Illegal PstI site found at 1188
Illegal NgoMIV site found at 388
Illegal NgoMIV site found at 408
Illegal NgoMIV site found at 424
Illegal NgoMIV site found at 889
Illegal NgoMIV site found at 1173 - 1000COMPATIBLE WITH RFC[1000]
Meško M, Lebar T, Dekleva P, Jerala R, Benčina M. Engineering and Rewiring of a Calcium-Dependent Signaling Pathway. ACS Synth Biol. 2020 Aug 21;9(8):2055-2065. doi: 10.1021/acssynbio.0c00133. Epub 2020 Jul 20. PMID: 32643923; PMCID: PMC7467823.