Difference between revisions of "Part:BBa K5301015"
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<partinfo>BBa_K5301015 short</partinfo> | <partinfo>BBa_K5301015 short</partinfo> | ||
− | NW50 is high molecular weight membrane scaffold protein used to produce large nanodiscs.spNW50 could construct nanodiscs with diameter | + | NW50 is high molecular weight membrane scaffold protein used to produce large nanodiscs.spNW50 could construct nanodiscs with large diameter, and is circularized by SpyTag-Spycatcher. |
+ | <center><html>Figure 1. Electron microscopic images of nanodisks with a particle size of about 100nm prepared by NW50. The particle size is in agreement with our DLS results.</html></center> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 01:15, 30 September 2024
spNW50 is high molecular weight membrane scaffold protein used to produce large nanodiscs.
NW50 is high molecular weight membrane scaffold protein used to produce large nanodiscs.spNW50 could construct nanodiscs with large diameter, and is circularized by SpyTag-Spycatcher.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 496
Illegal EcoRI site found at 1576
Illegal SpeI site found at 448
Illegal PstI site found at 664
Illegal PstI site found at 1204
Illegal PstI site found at 1237
Illegal PstI site found at 1744 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 496
Illegal EcoRI site found at 1576
Illegal NheI site found at 64
Illegal SpeI site found at 448
Illegal PstI site found at 664
Illegal PstI site found at 1204
Illegal PstI site found at 1237
Illegal PstI site found at 1744 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 496
Illegal EcoRI site found at 1576
Illegal BglII site found at 924
Illegal BglII site found at 1464
Illegal BglII site found at 1632
Illegal BamHI site found at 97 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 496
Illegal EcoRI site found at 1576
Illegal SpeI site found at 448
Illegal PstI site found at 664
Illegal PstI site found at 1204
Illegal PstI site found at 1237
Illegal PstI site found at 1744 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 496
Illegal EcoRI site found at 1576
Illegal SpeI site found at 448
Illegal PstI site found at 664
Illegal PstI site found at 1204
Illegal PstI site found at 1237
Illegal PstI site found at 1744 - 1000COMPATIBLE WITH RFC[1000]