Difference between revisions of "Part:BBa K5301015"

 
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<partinfo>BBa_K5301015 short</partinfo>
 
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NW50 is high molecular weight membrane scaffold protein used to produce large nanodiscs.spNW50 could construct nanodiscs with diameter of 100nm, and is circularized by SpyTag-Spycatcher.
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NW50 is high molecular weight membrane scaffold protein used to produce large nanodiscs.spNW50 could construct nanodiscs with large diameter, and is circularized by SpyTag-Spycatcher.
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<center><html>Figure 1. Electron microscopic images of nanodisks with a particle size of about 100nm prepared by NW50. The particle size is in agreement with our DLS results.</html></center>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 01:15, 30 September 2024


NW50 is high molecular weight membrane scaffold protein used to produce large nanodiscs.

NW50 is high molecular weight membrane scaffold protein used to produce large nanodiscs.spNW50 could construct nanodiscs with large diameter, and is circularized by SpyTag-Spycatcher.

Figure 1. Electron microscopic images of nanodisks with a particle size of about 100nm prepared by NW50. The particle size is in agreement with our DLS results.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 496
    Illegal EcoRI site found at 1576
    Illegal SpeI site found at 448
    Illegal PstI site found at 664
    Illegal PstI site found at 1204
    Illegal PstI site found at 1237
    Illegal PstI site found at 1744
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 496
    Illegal EcoRI site found at 1576
    Illegal NheI site found at 64
    Illegal SpeI site found at 448
    Illegal PstI site found at 664
    Illegal PstI site found at 1204
    Illegal PstI site found at 1237
    Illegal PstI site found at 1744
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 496
    Illegal EcoRI site found at 1576
    Illegal BglII site found at 924
    Illegal BglII site found at 1464
    Illegal BglII site found at 1632
    Illegal BamHI site found at 97
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 496
    Illegal EcoRI site found at 1576
    Illegal SpeI site found at 448
    Illegal PstI site found at 664
    Illegal PstI site found at 1204
    Illegal PstI site found at 1237
    Illegal PstI site found at 1744
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 496
    Illegal EcoRI site found at 1576
    Illegal SpeI site found at 448
    Illegal PstI site found at 664
    Illegal PstI site found at 1204
    Illegal PstI site found at 1237
    Illegal PstI site found at 1744
  • 1000
    COMPATIBLE WITH RFC[1000]