Difference between revisions of "Part:BBa K191004"

Revision as of 16:30, 21 October 2009

TRP promoter - RBS - RFP - Term

Part BBa_K191004 consists of the Trp promoter followed by the RFP gene. In E. coli, the Trp promoter is situated in front of the Trp operon, which contains the genes necessary for the tryptophan biosynthetic pathway. In brief, genes placed after the Trp promoter should be repressed in the presence of tryptophan.

Usage and Biology

This part permits characterization of part BBa_K191003 by fluorescence intensity. For complete genetic circuit, see Part:BBa_K191003:mainpage

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 43
Illegal SpeI site found at 51
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 43
Illegal SpeI site found at 51
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 43
Illegal SpeI site found at 51
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 43
Illegal SpeI site found at 51
Illegal AgeI site found at 666
Illegal AgeI site found at 778
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K191004 short</partinfo>
-Readout 1, RFP's transcription controlled by TRP operon
+Part BBa_K191004 consists of the Trp promoter followed by the RFP gene. In E. coli, the Trp promoter is situated in front of the Trp operon, which contains the genes necessary for the tryptophan biosynthetic pathway. In brief, genes placed after the Trp promoter should be repressed in the presence of tryptophan.
-Our first read-out system consists of the Trp promoter followed by the RFP gene. In E. coli, the Trp promoter is situated in front of the Trp operon, which contains the genes necessary for the tryptophane biosynthetic pathway. In brief, genes placed after the Trp promoter should be repressed in the presence of tryptophane.
-<font size="3">'''Read-Out n°1 BioBrick'''</font>
-This BioBrick was synthesized using the protocol we developed with the Klenow fragment. To do this, two long primers which together contained the sequence for the Trp promoter were ordered; these primers could self-anneal in the reaction mix, and the second strands that were missing at the extremities of the primers were fully synthesized using the Klenow fragment, instead of a classic extension using the TAQ DNA Polymerase.
-For more information, here is the [http://2009.igem.org/wiki/index.php?title=Team:EPF-Lausanne/Protocols/Klenow complete protocol].
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+This part permits characterization of part <partinfo>BBa_K191003</partinfo> by fluorescence intensity. For complete genetic circuit, see [[Part:BBa_K191003:mainpage]]
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