Difference between revisions of "Part:BBa K5246008"

 
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<partinfo>BBa_K5246008 short</partinfo>
 
<partinfo>BBa_K5246008 short</partinfo>
  
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"HfsH encodes a cytoplasmic deacetylase of 257 amino acids long. Deacetylase belongs to carbohydrate esterase family 4, catalyze the hydrolysis of N-linked acetyl groups from GlcNAc residues. C.Crescentus HfsH mutants were completely devoid of holdfast material
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===Introduction===
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===Usage and Biology===
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HfsH encodes a cytoplasmic deacetylase of 257 amino acids long. Deacetylase belongs to carbohydrate esterase family 4, catalyze the hydrolysis of N-linked acetyl groups from GlcNAc residues. C.Crescentus HfsH mutants were completely devoid of holdfast material
 
Another C. crescentus CB15 mutant, &#916;hfsH (YB2198), was used to study the role of deacethylation in adhesion efficiency.
 
Another C. crescentus CB15 mutant, &#916;hfsH (YB2198), was used to study the role of deacethylation in adhesion efficiency.
 
Indeed, this mutant is lacking the gene hfsH, encoding a deacetylase that affects both cohesive and adhesive properties of the holdfast.30 C. crescentus &#916;hfsH produces smaller holdfasts compared to the wild-type and the &#916;hfaB strains. These fully acetylated holdfasts are not anchored properly to the cell envelope and are shed in the medium. [2]
 
Indeed, this mutant is lacking the gene hfsH, encoding a deacetylase that affects both cohesive and adhesive properties of the holdfast.30 C. crescentus &#916;hfsH produces smaller holdfasts compared to the wild-type and the &#916;hfaB strains. These fully acetylated holdfasts are not anchored properly to the cell envelope and are shed in the medium. [2]
 
The main strain used in this study was Caulobacter crescentus CB15 &#916;hfaB (YB4251),29 a mutant strain from C. crescentus CB15 wild-type
 
The main strain used in this study was Caulobacter crescentus CB15 &#916;hfaB (YB4251),29 a mutant strain from C. crescentus CB15 wild-type
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(YB135). This mutant has a clean deletion of the hfaB gene and therefore does not synthesize HfaB, one of the holdfast anchor proteins. This strain still produces a holdfast, but is unable to anchor it to the cell envelope. As a consequence, the newly synthesized holdfast is shed in the culture medium and on surfaces. [3]"
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(YB135). This mutant has a clean deletion of the hfaB gene and therefore does not synthesize HfaB, one of the holdfast anchor proteins. This strain still produces a holdfast, but is unable to anchor it to the cell envelope. As a consequence, the newly synthesized holdfast is shed in the culture medium and on surfaces. [3]
  
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===Sequence and Features===
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K5246008 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5246008 SequenceAndFeatures</partinfo>
  
  
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K5246008 parameters</partinfo>
 
<partinfo>BBa_K5246008 parameters</partinfo>
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===Experimental characterization===
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===References===

Revision as of 13:13, 22 September 2024


CB2/CB2A HfsH Deacetylase

Introduction

Usage and Biology

HfsH encodes a cytoplasmic deacetylase of 257 amino acids long. Deacetylase belongs to carbohydrate esterase family 4, catalyze the hydrolysis of N-linked acetyl groups from GlcNAc residues. C.Crescentus HfsH mutants were completely devoid of holdfast material Another C. crescentus CB15 mutant, ΔhfsH (YB2198), was used to study the role of deacethylation in adhesion efficiency. Indeed, this mutant is lacking the gene hfsH, encoding a deacetylase that affects both cohesive and adhesive properties of the holdfast.30 C. crescentus ΔhfsH produces smaller holdfasts compared to the wild-type and the ΔhfaB strains. These fully acetylated holdfasts are not anchored properly to the cell envelope and are shed in the medium. [2] The main strain used in this study was Caulobacter crescentus CB15 ΔhfaB (YB4251),29 a mutant strain from C. crescentus CB15 wild-type (YB135). This mutant has a clean deletion of the hfaB gene and therefore does not synthesize HfaB, one of the holdfast anchor proteins. This strain still produces a holdfast, but is unable to anchor it to the cell envelope. As a consequence, the newly synthesized holdfast is shed in the culture medium and on surfaces. [3]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 10
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 10
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 10
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 10
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 10
    Illegal NgoMIV site found at 68
    Illegal NgoMIV site found at 92
    Illegal NgoMIV site found at 96
    Illegal NgoMIV site found at 169
    Illegal NgoMIV site found at 295
    Illegal NgoMIV site found at 456
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

Experimental characterization

References