Difference between revisions of "Part:BBa K5301006"
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In order to produce large nanodiscs more conveniently, we hope to flexibly extend the length of MSP according to demand, and thus propose the concept of multi-polymer MSP, which refers to large circular MSPs through end-to-end connections of multiple MSP fragments. | In order to produce large nanodiscs more conveniently, we hope to flexibly extend the length of MSP according to demand, and thus propose the concept of multi-polymer MSP, which refers to large circular MSPs through end-to-end connections of multiple MSP fragments. | ||
We used NW15 as the basic MSP and selected three types of linkers (Spy/Sdy/Snoop) to achieve the connection of different MSP fragments through the formation of covalent bonds, and adopted rigorous design to prevent self-cyclization of each fragment of the multi-polymer MSP. Finally, the successful cyclization of large circular MSPs is characterized by the fluorescence of mCherry after the combination of mCherry [1-10] and mCherry [11]. | We used NW15 as the basic MSP and selected three types of linkers (Spy/Sdy/Snoop) to achieve the connection of different MSP fragments through the formation of covalent bonds, and adopted rigorous design to prevent self-cyclization of each fragment of the multi-polymer MSP. Finally, the successful cyclization of large circular MSPs is characterized by the fluorescence of mCherry after the combination of mCherry [1-10] and mCherry [11]. | ||
| − | SnCSdT | + | SnCSdT, the second component of multi-polymerized MSP, is a fusion protein composed of SnoopCatcher, NW15, and SdyTag, with flexible GS linkers used to connect each part. |
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Revision as of 12:11, 30 September 2024
SnCSdT is one of the components of multi-polymerized MSP.
In order to produce large nanodiscs more conveniently, we hope to flexibly extend the length of MSP according to demand, and thus propose the concept of multi-polymer MSP, which refers to large circular MSPs through end-to-end connections of multiple MSP fragments. We used NW15 as the basic MSP and selected three types of linkers (Spy/Sdy/Snoop) to achieve the connection of different MSP fragments through the formation of covalent bonds, and adopted rigorous design to prevent self-cyclization of each fragment of the multi-polymer MSP. Finally, the successful cyclization of large circular MSPs is characterized by the fluorescence of mCherry after the combination of mCherry [1-10] and mCherry [11]. SnCSdT, the second component of multi-polymerized MSP, is a fusion protein composed of SnoopCatcher, NW15, and SdyTag, with flexible GS linkers used to connect each part.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1021
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1021
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 203
Illegal BglII site found at 575 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1021
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1021
Illegal AgeI site found at 100
Illegal AgeI site found at 860 - 1000COMPATIBLE WITH RFC[1000]