Difference between revisions of "Part:BBa K5078000"
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This is the Nitrous Oxide Reductase gene from P. stutzeri codon optimized for expression in Chlamydomonas reinhardtii
 
This is the Nitrous Oxide Reductase gene from P. stutzeri codon optimized for expression in Chlamydomonas reinhardtii
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<html><img src="https://static.igem.wiki/teams/5078/plasmid-pictures/nosz-p-stutzerii-l0-plasmid-picture.webp" width="400" height="auto"/></html><br>
 
  
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<html><div style="text-align: center;">
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===Usage and Biology===
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<img src="https://static.igem.wiki/teams/5078/plasmid-pictures/nosz-p-stutzerii-l0-plasmid-picture.webp" width="400" height="auto"/><br>
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Figure 1. NosZ P.stutzeri in a level 0 plasmid for later golden gate assembly.</div></html>
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===Verification of nosZ P. stu===
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Successful transformation of pL0-P.stu into host bacterium can be determined by a restriction digestion with the restriction enzyme BbsI, with the molecular weights being 1914bp and 2088bp. Additionally bacterial colonies should appear white in the present X-gal.
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<html><div style="text-align: center;">
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<img src="https://static.igem.wiki/teams/5078/experiments/digest-of-pstu-and-ddenit-l0.png" width="400" height="auto"/><br>
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Figure 2. pL0-NosZ-P.stuzeri diagnostic digest using BbsI on a 8% agarose gel. The restriction digest indicated that the two colonies taken from both culture 1 and 2 all have pL0-NosZ-P.stuzeri.</div></html>
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===Structure simulation===
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<html><div style="text-align: center;">
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<img src="https://static.igem.wiki/teams/5078/modeling/p-stu-nosz-folding-sphere.webp" width="400" height="auto"/><br>
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Figure 3. Structure prediction of nitrous oxide reductase. The structure has two identical 65.8kDa subunits that each contain 6 copper atoms</div></html>
  
 
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Revision as of 01:03, 22 September 2024


Nos Z gene from P. stutzeri

This is the Nitrous Oxide Reductase gene from P. stutzeri codon optimized for expression in Chlamydomonas reinhardtii


Figure 1. NosZ P.stutzeri in a level 0 plasmid for later golden gate assembly.

Verification of nosZ P. stu

Successful transformation of pL0-P.stu into host bacterium can be determined by a restriction digestion with the restriction enzyme BbsI, with the molecular weights being 1914bp and 2088bp. Additionally bacterial colonies should appear white in the present X-gal.


Figure 2. pL0-NosZ-P.stuzeri diagnostic digest using BbsI on a 8% agarose gel. The restriction digest indicated that the two colonies taken from both culture 1 and 2 all have pL0-NosZ-P.stuzeri.

Structure simulation


Figure 3. Structure prediction of nitrous oxide reductase. The structure has two identical 65.8kDa subunits that each contain 6 copper atoms

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 541
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1303
    Illegal PstI site found at 541
    Illegal NotI site found at 145
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1520
    Illegal BamHI site found at 467
    Illegal XhoI site found at 34
    Illegal XhoI site found at 1588
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 541
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 541
    Illegal NgoMIV site found at 1075
  • 1000
    COMPATIBLE WITH RFC[1000]