Difference between revisions of "Part:BBa K5061005"
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This Golden Gate adapter contains two BsaI recognition sites in opposite direction. | This Golden Gate adapter contains two BsaI recognition sites in opposite direction. | ||
| − | It is present in our composite parts [[Part:BBa_K5061031|BBa_K5061031]] and [[Part:BBa_K5061131|BBa_K5061131]]and allows rapid engineering using Golden Gate Assembly of helper phages (HP) for phage-assisted continuous and non-continuous evolution (PACE and PANCE). | + | It is present in our composite parts [[Part:BBa_K5061031|BBa_K5061031]] and [[Part:BBa_K5061131|BBa_K5061131]] and allows rapid engineering using Golden Gate Assembly of helper phages (HP) for phage-assisted continuous and non-continuous evolution (PACE and PANCE). |
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Latest revision as of 22:13, 20 September 2024
Golden Gate adapter for Helper phages (HP) M13KO7-ΔgVI and M13KO7-ΔgIII-ΔgVI Golden G
This Golden Gate adapter contains two BsaI recognition sites in opposite direction.
It is present in our composite parts BBa_K5061031 and BBa_K5061131 and allows rapid engineering using Golden Gate Assembly of helper phages (HP) for phage-assisted continuous and non-continuous evolution (PACE and PANCE).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 12
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 18
Illegal BsaI.rc site found at 6