Difference between revisions of "Part:BBa K5396001"
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=== Usage and Biology === | === Usage and Biology === | ||
| − | + | <p>Metodo de criação da barbie, importancia da rfp e da tag mad10.</p> | |
| + | https://static.igem.wiki/teams/5396/registry/imagem-2024-09-20-141552930.png | ||
| + | <p style="font-size: 11px;"><b>Figure 1.</b> AlphaFold 3 3D simulation of Barbie1 protein with miRFP and three Mad10 tags. | ||
| + | </p> | ||
Revision as of 18:23, 20 September 2024
Barbie1_RFP_3xMad10
BARBIE1 is a synthetic protein derived from BaCBM2 through a process of reverse engineering. It has the increased ability to bind to plastics when compared to BaCBM2.
The BARBIE1 protein is fused with the red fluorescent protein (RFP)[ ], which exhibits an excitation maximum at 558 nm and an emission maximum at 583 nm. This fusion enhances the visualization of BARBIE1 by fluorescence-based methods.
This part was used as template to construct BBa_K5396004.
Usage and Biology
Metodo de criação da barbie, importancia da rfp e da tag mad10.
Figure 1. AlphaFold 3 3D simulation of Barbie1 protein with miRFP and three Mad10 tags.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 594
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 88
- 1000COMPATIBLE WITH RFC[1000]