Difference between revisions of "Part:BBa K5396001"
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This part was used as template to construct BBa_K5396004.
 
This part was used as template to construct BBa_K5396004.
  
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=== Usage and Biology ===
  
  

Revision as of 18:21, 20 September 2024


Barbie1_RFP_3xMad10

BARBIE1 is a synthetic protein derived from BaCBM2 through a process of reverse engineering. It has the increased ability to bind to plastics when compared to BaCBM2.

The BARBIE1 protein is fused with the red fluorescent protein (RFP)[ ], which exhibits an excitation maximum at 558 nm and an emission maximum at 583 nm. This fusion enhances the visualization of BARBIE1 by fluorescence-based methods.

This part was used as template to construct BBa_K5396004.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 594
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 88
  • 1000
    COMPATIBLE WITH RFC[1000]