Difference between revisions of "Part:BBa K5396006"
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===Usage and Biology===
 
===Usage and Biology===
−
texto sobre a spidroina e barbie
+
 
 +
<p>Spidroins are the primary proteins that compose spider silk. This part contains the N-terminal domain, which s involved in the initial formation of silk fibers and is crucial for the protein's solubility and stability.
  
 
===Part Generation===
 
===Part Generation===

Revision as of 16:33, 20 September 2024


T7-Nt-BaCBM2-Cys

This part is the N-terminal of Spidroin fused with our BARBIE1 protein with an additional cysteine (BBa_K5396004).

Usage and Biology

Spidroins are the primary proteins that compose spider silk. This part contains the N-terminal domain, which s involved in the initial formation of silk fibers and is crucial for the protein's solubility and stability.

Part Generation

Gibson Assembly

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 96
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 30
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 96
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 96
  • 1000
    COMPATIBLE WITH RFC[1000]