Difference between revisions of "Part:BBa K5396005"

Line 6: Line 6:
  
 
===Usage and Biology===
 
===Usage and Biology===
 
+
texto sobre spidroina, texto sobre bacbm2
 
===Part Generation===
 
===Part Generation===
 
Gibson assembly
 
Gibson assembly

Revision as of 16:25, 20 September 2024


T7-Nt2RepCt

This part is the N-terminal of Spidroin fused to our BaCBM2 with an additional cystein (BBa_K5396003).

Usage and Biology

texto sobre spidroina, texto sobre bacbm2

Part Generation

Gibson assembly

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 96
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 30
    Illegal XhoI site found at 1148
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 96
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 96
  • 1000
    COMPATIBLE WITH RFC[1000]