Difference between revisions of "Part:BBa K5115065"
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===Characterization=== | ===Characterization=== | ||
| + | ====Agarose gel electrophoresis==== | ||
| + | {| | ||
| + | | <html><img style="width:400px" src="https://static.igem.wiki/teams/5115/registry/cso-s3.png" alt="contributed by Fudan iGEM 2024"></html> | ||
| + | |- | ||
| + | | '''Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures. | ||
| + | M: DNA Marker; Lanes 1-8: Amplification of specific regions corresponding to ''csoS2'', ''csoS3'', ''csoS4A'', ''csoS4B'', ''csoS1C'', ''csoS1A'', ''csoS1B'', and ''csoS1D'', demonstrating the presence of the expected subunits derived from the carboxysome plasmid without the csoS3 component. | ||
| + | ''' | ||
| + | |} | ||
| + | |||
| + | ===Sequence and Features=== | ||
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Revision as of 18:56, 27 September 2024
cso, without csoS3
Contents
Introduction
Usage and Biology
Characterization
Agarose gel electrophoresis
|
| Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.
M: DNA Marker; Lanes 1-8: Amplification of specific regions corresponding to csoS2, csoS3, csoS4A, csoS4B, csoS1C, csoS1A, csoS1B, and csoS1D, demonstrating the presence of the expected subunits derived from the carboxysome plasmid without the csoS3 component. |
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 133
Illegal NotI site found at 5051 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 291
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 799
Illegal AgeI site found at 1750
Illegal AgeI site found at 2431
Illegal AgeI site found at 3385 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 191