Difference between revisions of "Part:BBa K283010"
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This part codes for Streptavidin which binds with biotin. The Streptavidin coding region does not contain any rbs nor terminator.This part does not pose any biological threat. | This part codes for Streptavidin which binds with biotin. The Streptavidin coding region does not contain any rbs nor terminator.This part does not pose any biological threat. | ||
| + | The UGent Belgium 2016 iGEM team improved this part by making it more practical for use in fusion proteins. The new part, [[Part:BBa_K1896000|BBa_K1896000]], codes for a monomeric Streptavidin mutant, contains an upstream peptide linker and is compatible with the RFC25 "Freiburg standard". | ||
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Latest revision as of 20:05, 18 October 2016
Streptavidin
This part codes for Streptavidin which binds with biotin. The Streptavidin coding region does not contain any rbs nor terminator.This part does not pose any biological threat.
The UGent Belgium 2016 iGEM team improved this part by making it more practical for use in fusion proteins. The new part, BBa_K1896000, codes for a monomeric Streptavidin mutant, contains an upstream peptide linker and is compatible with the RFC25 "Freiburg standard".
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 43
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 85
Illegal AgeI site found at 136 - 1000COMPATIBLE WITH RFC[1000]