Difference between revisions of "Part:BBa K5492732"

Revision as of 21:00, 25 September 2024

S1_aptamer_reverse_complement_strand

ssDNA sequence designed to be complementary for the 5' end of s1_aptamer_with_fluorophore, it contains a quencher molecule (3'Iowa Black® RQ) at its 3' end, which absorbs the light emitted by the 5' fluorophore molecule (5' Cy5™) of the aptamer until it binds histamine.

Usage and Biology

Aptamers are generally artificial ssDNA, RNA, or peptide oligomers which bind to specific target molecules. All ssDNA aptamers we utilise are proven to be able to bind specifically to histamine, thus preventing the binding of the molecule to a histamine receptor.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K5492732 short</partinfo>
-ssDNA sequence designed to be complementary for the 5' end of [https://parts.igem.org/Part:BBa_K5492722 s1_aptamer_with_fluorophore], it contains a quencher molecule which absorbs the light emitted by fluorophore at its 3' end, until the aptamer binds histamine.
+ssDNA sequence designed to be complementary for the 5' end of [https://parts.igem.org/Part:BBa_K5492722 s1_aptamer_with_fluorophore], it contains a quencher molecule (3'Iowa Black® RQ) at its 3' end, which absorbs the light emitted by the 5' fluorophore molecule (5' Cy5™) of the aptamer until it binds histamine.
@@ Line 11: / Line 12: @@
 <!-- -->
-<span class='h3bb'>Sequence and Features</span>
+===<span class='h3bb'>Sequence and Features</span>===
+https://static.igem.wiki/teams/5492/registry/aptamer-fluorophore.jpg
 <partinfo>BBa_K5492732 SequenceAndFeatures</partinfo>