Difference between revisions of "Part:BBa K191008:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
 
Site specific mutation (a.a#427 ILE -> PHE) on plasmid with part <partinfo>BBa_K191003</partinfo>.
 
Site specific mutation (a.a#427 ILE -> PHE) on plasmid with part <partinfo>BBa_K191003</partinfo>.
 +
 +
Primers ordered for mutagenesis experiments:
 +
 +
Forward primer: 5'-ggttgccagataatccctttatattcgcgtccgat-3'
 +
 +
Reverse primer: 5'-atcggacgcgaatataaagggattatctggcaacc-3'
  
 
===Source===
 
===Source===

Latest revision as of 14:34, 21 October 2009

Promoter LacI - RBS - LovTAP(ILE427PHE) - Term


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 370
    Illegal PstI site found at 627
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 370
    Illegal PstI site found at 627
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 370
    Illegal PstI site found at 627
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 370
    Illegal PstI site found at 627
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 764


Design Notes

Site specific mutation (a.a#427 ILE -> PHE) on plasmid with part BBa_K191003.

Primers ordered for mutagenesis experiments:

Forward primer: 5'-ggttgccagataatccctttatattcgcgtccgat-3'

Reverse primer: 5'-atcggacgcgaatataaagggattatctggcaacc-3'

Source

Mutagenesis kit of Stratagene was used.

BBa_K191003

References