Difference between revisions of "Part:BBa K5492701"
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J1 ssDNA sequence designed for serving as connector between avidin-coated magnetic beads and the 5' overhang of [https://parts.igem.org/Part:BBa_K5492711 J1_aptamer] and [https://parts.igem.org/Part:BBa_K5492710 Y1_aptamer]. | J1 ssDNA sequence designed for serving as connector between avidin-coated magnetic beads and the 5' overhang of [https://parts.igem.org/Part:BBa_K5492711 J1_aptamer] and [https://parts.igem.org/Part:BBa_K5492710 Y1_aptamer]. | ||
| − | The connector sequence contains a 5' biotin tag that lets the sequence bind to the bead via avidin-biotin connection. | + | The connector sequence contains a 5' biotin tag (5'biosg) that lets the sequence bind to the bead via avidin-biotin connection. |
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| − | <span class='h3bb'>Sequence and Features</span> | + | ===<span class='h3bb'>Sequence and Features</span>=== |
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| + | https://static.igem.wiki/teams/5492/registry/aptamer-beads-illustr-small.jpg | ||
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<partinfo>BBa_K5492701 SequenceAndFeatures</partinfo> | <partinfo>BBa_K5492701 SequenceAndFeatures</partinfo> | ||
Revision as of 20:22, 25 September 2024
J1_Y1_aptamer_connector
J1 ssDNA sequence designed for serving as connector between avidin-coated magnetic beads and the 5' overhang of J1_aptamer and Y1_aptamer. The connector sequence contains a 5' biotin tag (5'biosg) that lets the sequence bind to the bead via avidin-biotin connection.
Usage and Biology
Aptamers are generally artificial ssDNA, RNA, or peptide oligomers which bind to specific target molecules. All ssDNA aptamers we utilise are proven to be able to bind specifically to histamine, thus preventing the binding of the molecule to a histamine receptor.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]