Difference between revisions of "Part:BBa K5115063"
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__TOC__
 
__TOC__
 
===Introduction===
 
===Introduction===
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This composite part combines all the subunit of the hydrogenase in our ribozyme-assisted polycistronic co-expression system：pRAP. It's worth mention that, in this part, the subunit hoxF is fused with EP, check  [https://parts.igem.org/Part:BBa_K51150058 BBa_K51150058(hoxF fused with EP)]for more details. To learn more about the hydrogenase, please check [https://parts.igem.org/Part:BBa_K51150020 BBa_K51150020(hox and hyp operon)]. To get more information about pRAP, please check [https://2022.igem.wiki/fudan/parts part wiki of 2022 Fudan iGEM ].
  
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===Usage and Biology===
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===Usage and biology===
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This part is crucial in  [https://parts.igem.org/Part:BBa_K51150067 BBa_K51150067(mineral, F module)], click on the link to view the experiment details.
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===Characterization===
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Revision as of 03:37, 29 September 2024


hox and hyp, with EP targeted hoxF

contributed by Fudan iGEM 2023

Introduction

This composite part combines all the subunit of the hydrogenase in our ribozyme-assisted polycistronic co-expression system：pRAP. It's worth mention that, in this part, the subunit hoxF is fused with EP, check BBa_K51150058(hoxF fused with EP)for more details. To learn more about the hydrogenase, please check BBa_K51150020(hox and hyp operon). To get more information about pRAP, please check part wiki of 2022 Fudan iGEM .

Usage and biology

This part is crucial in BBa_K51150067(mineral, F module), click on the link to view the experiment details.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 485
    Illegal BglII site found at 563
    Illegal BglII site found at 9209
    Illegal BglII site found at 10027
    Illegal BglII site found at 10320
    Illegal BamHI site found at 956
    Illegal XhoI site found at 493
    Illegal XhoI site found at 685
    Illegal XhoI site found at 1163
    Illegal XhoI site found at 3402
    Illegal XhoI site found at 5231
    Illegal XhoI site found at 6393
    Illegal XhoI site found at 8232
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1256
    Illegal NgoMIV site found at 1936
    Illegal NgoMIV site found at 4543
    Illegal NgoMIV site found at 4650
    Illegal NgoMIV site found at 4920
    Illegal NgoMIV site found at 5766
    Illegal NgoMIV site found at 5998
    Illegal AgeI site found at 446
    Illegal AgeI site found at 5718
    Illegal AgeI site found at 11103
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 267
    Illegal BsaI site found at 429
    Illegal BsaI site found at 3056
    Illegal BsaI.rc site found at 9257
    Illegal BsaI.rc site found at 9761
    Illegal SapI.rc site found at 378


References