Difference between revisions of "Part:BBa K5115058"
 
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__TOC__
 
__TOC__
 
===Introduction===
 
===Introduction===
 +
Combine hoxF directly with EP. The hoxF is a subunit of the hydrogenase, check it in [https://parts.igem.org/Part:BBa_K5115020 BBa_K5115020(hox and hyp operon)]. EP can direct external proteins into carboxysomes, check it in [https://parts.igem.org/Part:BBa_K51150002 BBa_K51150002(EP)].
  
 
===Usage and Biology===
 
===Usage and Biology===
−
 
+
Binding hoxF with EP can direct the hydrogenase into the carboxysome, creating a safe and stable environment for the enzyme to work.
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===Characterization===
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Latest revision as of 01:44, 29 September 2024


hoxF fused with EP

contributed by Fudan iGEM 2023

Introduction

Combine hoxF directly with EP. The hoxF is a subunit of the hydrogenase, check it in BBa_K5115020(hox and hyp operon). EP can direct external proteins into carboxysomes, check it in BBa_K51150002(EP).

Usage and Biology

Binding hoxF with EP can direct the hydrogenase into the carboxysome, creating a safe and stable environment for the enzyme to work.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 577
    Illegal BglII site found at 1395
    Illegal BglII site found at 1688
    Illegal XhoI site found at 22
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 456
    Illegal NgoMIV site found at 994
    Illegal NgoMIV site found at 1204
    Illegal NgoMIV site found at 1516
    Illegal AgeI site found at 2471
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 625
    Illegal BsaI.rc site found at 1129


References